![]() The images were captured at 60X magnification. ![]() ![]() Panel e represents control cells with no primary antibody to assess background. Panel d represents the merged image showing cytoplasmic localization. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). The cells were labeled with SHIP1 Rabbit Monoclonal Antibody (T.7.7) (Product # MA5-14893) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Immunofluorescence analysis of SHIP1 was performed using 70% confluent log phase THP-1 cells.
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